Western Province Womens duo Faye Tunnicliffe and Dane van Niekerk have been overlooked after Cricket SA named their 15-player contracted list in the build-up to the ICC Womens World Cup in India later this year.
Fellow Province pair Lara Goodall and Delmi Tucker have also lost their national contracts after being part of last years group.
Both players have struggled with injuries over the last 18 months, although Goodall made her international comeback during the ODI series against England at the end of last year.
The left-hand top-order batter did not make full use of her opportunities with scores of 7, 31 and 17 in her three matches.
Tucker, meanwhile, last played for the Proteas in the once-off Test against India last year in Chennai when she unfortunately bagged a pair of ducks.