Barbora Krejcikova made a tearful Wimbledon exit as the defending champion crashed to a three-set defeat against Emma Navarro, while mens top seed Jannik Sinner crushed Pedro Martinez to reach the last 16 on Saturday.
Krejcikova appeared to be struggling with injury as she wept in the closing stages of the third-round clash on Court One.
Navarro took advantage to cause the latest upset in the womens tournament following the exits of five of the top six seeds.
Krejcikova had to fight back from a set down to beat rising star Alexandra Eala in the first round before another tense three-set win over Caroline Dolehide in the second round.
There would be no dramatic escape for Krejcikova this time, with the 17th seeds fitness problems finally catching up with her against American 10th seed Navarro.