Holders Edo Queens fell to a 1-0 defeat against Remo Stars Ladies in their opening fixture of the Nigeria Women Football League, NWFL, Super Six playoff.
Olasubomi Ogundele scored the winning goal two minutes from time.
It was Remo Stars' first-ever victory over Moses Aduku's side.
Former champions Bayelsa Queens got their campaign off to a winning start with a 1-0 victory over Rivers Angels.
Alaba Olabiyi's 34th-minute strike separated both teams on the day.